Pesticide Residue Methods





Category Others
Class Name Pesticide Specific Enforcement Method, Azoxystrobin
Method Title Enforcement Method for the Determination of Residues of ICIA5504 and R230310 in Grain and Grapes for EPA Confirmation
Method Date 1995/05/01
Method Type Quantitative
Scope and Application Analysis for Azoxystrobin and related metabolite Analysis of Grain and Grapes Can be used with minor modifications for most crop matrices GC-NPD of HPLC-UV Analysis and Detection.
Method Summary ICIA5504 and R230310 residues in grain samples are extracted in 90:10/acetonitrile:water. An aliquot of the extract is cleaned up by adsorption chromatography on a Florisil column. The eluate is evaporated to dryness and taken up in a known volume of acetone for analysis by gas chromatography with nitrogen-phosphorus detection (GC-NPD). ICIA5504 and R230310 residues in grape samples are extracted in 90:10/acetonitrile:water. An aliquot of the extract is cleaned up by adsorption chromatography on a silica sorbent. The eluate is evaporated to dryness and taken up in a known volume of acetone for analysis by GC-NPD or in mobile phase for analysis by high performance liquid chromatography with ultra-violet detection (HPLC-UV).
Applicable Concentration Range Estimated LOQ: 0.02 - 0.05 mg/Kg
QC Requirements See attached method
Method Performance/Validation See attached method
Method Source US EPA and Zeneca Inc.
Method SOP PDF icon SOP 
Citation Zeneca Ag. Products Method RAM 243104