Pesticide Residue Methods





Category Others
Class Name Pesticide Specific Enforcement Method, Abamectin and Avermectins
Method Title DETERMINATION OF ABAMECTIN RESIDUES IN FRUITS AND VEGETABLES (RAW AGRICULTURAL COMMODITY) BY LC-MS/MS
Method Date 2008/08/20
Method Type Quantitative
Scope and Application Analysis for abamectin residues (avermectin B1a, avermectin B1b, and 8,9-Z avermectin B1a) Analysis of fruits and vegetables Can be used with minor modifications for most crop matrices LC-MS/MS
Method Summary The method described herein is capable of determining abamectin residues (avermectin B1a, avermectin B1b, and 8,9-Z avermectin B1a) in a variety of raw agricultural commodity (RAC) fruits and vegetables. It is based on Novartis Crop Protection, Inc. Method M-073.1 Residues of avermectin B1a, avermectin B1b, and 8,9-Z avermectin B1a are extracted from homogenized crop samples with acetonitrile:0.1pct H3PO4 (25:75, v/v). The isolated residues are then partitioned into hexane. A suitable aliquot of the hexane extract is purified using aminopropyl solid phase extraction (SPE) cleanup. The purified extract is evaporated to dryness, reconstituted in acetonitrile, then submitted to HPLC analysis. During routine analysis, determination and quantitation of all targeted analytes are conducted using HPLC employing mass spectrometric (MS/MS) detection (LC-MS/MS). The limit of quantitation (LOQ) for all three analytes, in all matrices, is 0.002 ppm.
Applicable Concentration Range Estimated LOQ: 2 ng/g
QC Requirements See Attached Method
Method Performance/Validation See Attached Method
Method Source IR-4
Method SOP PDF icon SOP 
Citation Morse Laboratories Inc. Analytical Method Meth-192, Revision 2