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Category |
Carbamates and pyrethroids
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Class Name |
Dithiocarbamate Pesticides
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Method Title |
Determination of Ethylene bis-Dithiocarbamate in Fruits and Vegetables by HPLC-FL
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Method Date |
2003/07/03
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Method Type |
Screening, Quantitative
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Scope and Application |
For the analysis of Ethylene bis-Dithiocarbamate (EBDC) in fruits and vegetables with a reporting limit of 0.04 microgram per gram as zineb equivalent.
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Method Summary |
A homogenized sample (50.0 grams) is extracted in 4N HCl and SnCl2 and then diluted in purified water before cleanup by ion exchange using resins in a BioRad Econo-Pak column. The analyte is eluted using NaHCO3 and derivatized with o-Phthaladehyde and
2-Mercaptoethanol involving overnight heating. The solution is further acidified with HCl and mixed well in methanol prior to analysis by HPLC/FL (excitation 290 nm, emission 447 nm). An ODS-1 analytical column (25 cm, 4.6 mm) is used for separation.
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Applicable Concentration Range |
Ethylene bis-Dithiocarbamates can be detected in fruits and vegetables between 0.02 and 0.21 microgram per grams.
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QC Requirements |
See uploaded SOP
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Method Performance/Validation |
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Method Source |
Canadian Food Inspection Agency
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Method SOP |
 SOP |
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Citation |
Canadian Food Inspection Agency, SPR-002-V2.4
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