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Category |
Other substances and contaminants
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Class Name |
Macrocyclic Lactone Insecticides, Avermectin Acaricides
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Method Title |
Determination of Abamectin in Fruits and Vegetables using HPLC with Fluorescence Detection
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Method Date |
2004/03/08
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Method Type |
Screening, Quantitative
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Scope and Application |
For determination of Abamectin (Avermectin B1) residues in fruits and vegetables at the reporting limit of 0.003 microgram per gram.
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Method Summary |
A thawed sample (10.0 g) is homogenized in acetonitrile, water and hexane and extracted repeatedly by centrifugation in hexane followed by cleanup on a Sep-Pak Vac aminopropyl (NH2) SPE column. The analyte is finally eluted into a silylated centrifuge
tube using acetone and methylene chloride. Derivatization is achieved using NMIM/ACN, TFAA/CAN and methanolic ammonium hydroxide. The sample is then diluted in methanol prior to analysis by HPLC-FL using a Zorbax, SB-C18, 4.6, 250 mm, 5 micrometre analytical
column for separation.
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Applicable Concentration Range |
Abamectin may be detected in fruits and vegetables in the concentration range 0.003 - 0.010 microgram per gram.
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QC Requirements |
See uploaded SOP
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Method Performance/Validation |
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Method Source |
Canadian Food Inspection Agency
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Method SOP |
 SOP |
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Citation |
Canadian Food Inspection Agency, SPR-007-V1.0
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