| Category |
Antibacterial substances, including sulfonamides and quinolones |
| Drug Class Name |
Aminoglycosides (AMG) |
| Method Title |
Confirmation of Aminoglycosides by HPLC-MS/MS |
| Method Date |
2011/06/03 |
| Method Type |
Screening, Confirmatory, Quantitative |
| Scope and Application |
This procedure is for the detection of aminoglycoside antibiotics (spectinomycin, hygromycin, streptomycin, dihydrostreptomycin, Amikacin, kanamycin, apramycin, tobramycin, gentamycin and neomycin) in bovine, porcine, and avian (poultry) liver, kidney, and muscle. |
| Method Summary |
Aminoglycoside (AMG) residues are extracted from tissue using buffer containing trichloroacetic acid as a protein precipitant. The extract is neutralized prior to solid-phase extraction (SPE) clean-up with a weak cation exchange cartridge followed by elution with acidic methanol. The methanolic extract is evaporated and reconstituted in an aqueous ion-pair reagent followed by ion-pair reversed-phase liquid chromatography with detection by electrospray ion trap mass spectrometry.
Confirmation is accomplished by comparison of retention time and full or partial scan MS/MS spectra with those of a fortified tissue standard and external standard respectively.
|
| Applicable Concentration Range |
Applicable concentration range for muscle is:
spectinomycin 250 µg kg-1
hygromycin 400 µg kg-1
streptomycin 400 µg kg-1
dihydrostreptomycin 400 µg kg-1
Amikacin 420 µg kg-1
kanamycin 400 µg kg-1
apramycin 100 µg kg-1
tobramycin 100 µg kg-1
gentamycin 100 µg kg-1
neomycin 100 µg kg-1
|
| QC Requirements |
QC requirements are defined in the SOP. |
| Method Source |
USDA-FSIS |
| Method SOP |
 SOP |
| Citation |
Citation |