| Category |
Antibacterial substances, including sulfonamides and quinolones |
| Drug Class Name |
Beta- Lactams: Selected penicillins and cephalosporins |
| Method Title |
Screening and Confirmation of ß-Lactam Antibiotics by HPLC-MS/MS |
| Method Date |
2011/07/02 |
| Method Type |
Screening, Confirmatory |
| Scope and Application |
The method is applicable for confirming presence of β-Lactam (ampicillin, nafcillin, cefazolin, desfuroylceftiofur cysteine disulfide metabolite of ceftiofur, penicillin G and desacetyl cephapirin) residues in bovine and porcine kidney and muscle. This method may also be used to screen for amoxicillin, cloxacillin, dicloxacillin and oxacillin in the same matrices. |
| Method Summary |
The β-Lactams are extracted from thawed lean tissues by homogenization and centrifugation using acetonitrile/water followed by clean-up using solid phase extraction. Penicillin V is used as an internal standard The final eluate is examined for presence of β-Lactams by LC/MS/MS using a triple quadrupole mass spectrometer under electrospray ionization conditions. A C18 waters, YMC ODS-AQ column is used for separation of the analytes. |
| Applicable Concentration Range |
The analytes may be detected in bovine and porcine kidney and muscle at the following concentration levels: ampicillin ≥ 10 µg kg-1; nafcillin ≥ 20 µg kg-1; cefazolin ≥ 50 µg kg-1, desfuroylceftiofur cysteine disulfide metabolite of ceftiofur ≥ 50 µg kg-1, penicillin G each ≥ 50 µg kg-1; and desacetyl cephapirin ≥ 100 µg kg-1. Screening of amoxicillin and cloxacillin at ≥10 µg kg-1 as well as dicloxacillin and oxacillin at ≥ 50 µg kg-1 is also possible. |
| QC Requirements |
See attached SOP |
| Method Source |
USDA-FSIS |
| Method SOP |
 SOP |
| Citation |
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