Veterinary Drugs Residue Methods


 



Category Antibacterial substances, including sulfonamides and quinolones
Drug Class Name Beta-Lactams, Selected penicillins and cephalosporins
Method Title Screening and Confirmation of Beta-Lactam Antibiotics by HPLC-MS/MS
Method Date 2011/07/02
Method Type Screening, Confirmatory
Scope and Application The method is applicable for confirming presence of Beta-Lactam (ampicillin, nafcillin, cefazolin, desfuroylceftiofur cysteine disulfide metabolite of ceftiofur, penicillin G and desacetyl cephapirin) residues in bovine and porcine kidney and muscle. This method may also be used to screen for amoxicillin, cloxacillin, dicloxacillin and oxacillin in the same matrices.
Method Summary The Beta-Lactams are extracted from thawed lean tissues by homogenization and centrifugation using acetonitrile/water followed by clean-up using solid phase extraction. Penicillin V is used as an internal standard The final eluate is examined for presence of Beta-Lactams by LCMSMS using a triple quadrupole mass spectrometer under electrospray ionization conditions. A C18 waters, YMC ODS-AQ column is used for separation of the analytes.
Applicable Concentration Range The analytes may be detected in bovine and porcine kidney and muscle at the following concentration levels namely ampicillin equal or greater than 10 µg kg-1, nafcillin equal or greater than 20 µg kg-1, cefazolin equal or greater than 50 µg kg-1, desfuroylceftiofur cysteine disulfide metabolite of ceftiofur equal or greater than 50 µg kg-1, penicillin G each equal or greater than 50 µg kg-1, and desacetyl cephapirin equal or greater than 100 µg kg-1. Screening of amoxicillin and cloxacillin at equal or greater than 10 µg kg-1 as well as dicloxacillin and oxacillin at equal or greater than 50 µg kg-1 is also possible.
QC Requirements See attached SOP
Method Source USDA-FSIS
Method SOP PDF icon SOP 
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