| Category |
Anticoccidials, including nitroimidazoles |
| Drug Class Name |
Narasin: Anticoccidial |
| Method Title |
Narasin in Cattle and Chicken Tissues |
| Method Date |
1991/07/01 |
| Method Type |
Screening |
| Scope and Application |
The method is suitable for screeining the presence of Narasin in bovine and chicken liver, kidney, skin and fat tissues. |
| Method Summary |
Ground or blended lean liver and kidney tissue are extracted in methanol by centrifugation and the supernatant further extracted in carbon tetrachloride. This is cleaned-up using a silica gel column and anhydrous sodium sulfate. Narasin is eluted from the column with chloroform:methanol(95:5) and evaporated to dryness and the residue redissolved in chloroform:methanol (99:l) prior to a second clean-up step using alumina column. Hexane is the added to remove fat tissue and after evaporation, the residue is dissolved in methanol before analysis by thin layer chromatography.The developing solvent was a mixture of carbon tetrachloride, benzene and ethylene glycol monomethyl ether. Ground or blended skin and fat tissue samples are redistilled in hexane and centrifuged. The supernate is then extracted in acetonitrile and cleaned–up as above. After evaporating the hexane layer, the residue is dissolve in methanol and analysed as above. |
| Applicable Concentration Range |
As low as 5 µg kg-1 narasin standard may be spotted (and viewed) on the plate. |
| QC Requirements |
See attached SOP |
| Method Source |
USDA-FSIS |
| Method SOP |
 SOP |
| Citation |
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