| Category |
Anticoccidials, including nitroimidazoles |
| Drug Class Name |
Nitorimidazoles |
| Method Title |
Confirmation of Nitroimidazoles by ESI-LC/MS/MS |
| Method Date |
2005/02/07 |
| Method Type |
Confirmatory, Quantitative |
| Scope and Application |
This method is suitable for the analysis/confirmation of hydroxy-dimetridazole and hydroxy-ipronidazole in swine and poultry muscle. |
| Method Summary |
Homogenized meat samples mixed with K2HPO4, NaCl and ethyl acetate are extracted by centrifugation after thorough shaking. The ethyl acetate is then evaporated. More ethyl acetate is added along with HCl and the extract mixed with hexane. After gentle swirling, the bottom aqueous layer is collected into a scintillation vial or small beaker while avoiding any emulsion. The extract pH is then adjusted to 4.8-5.2 followed by sample clean-up using a C18 SPE column. The final methanol eluate is evaporated to dryness and the residue dissolved in water before filtration through 0.22 μm syringe filter for analysis by LC-MS/MS in ESI MRM mode. A Phenomenex RP-C-18 analytical column is used |
| Applicable Concentration Range |
Hydroxy-dimetridazole and hydroxy-ipronidazole levels of ≥ 0.20 µg may be reliably confirmed using this method. |
| QC Requirements |
See attached SOP |
| Method Source |
USDA-FSIS |
| Method SOP |
 SOP |
| Citation |
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