| Category |
Antibacterial substances, including sulfonamides and quinolones |
| Drug Class Name |
Novobiocin and Virginiamycin: Antibiotics |
| Method Title |
Novobiocin and Virginiamycin |
| Method Date |
1991/07/01 |
| Method Type |
Screening, Quantitative |
| Scope and Application |
This method is applicable for analysis screening of the antibiotic Novobiocin and Virginiamycin in bovine, porcine, and avian liver, muscle, or kidney tissue. |
| Method Summary |
The analytes are extracted by centrifugation of homogenized tissue (bovine, porcine, and avian liver, muscle, or kidney tissue) in methanol. An aliquot (1 mL) of methanol is filtered using a 0.2 µm syringe filter for HPLC analysis of novobiocin. Using a second methanol aliquot (6 mL), viriginiamicin is extracted into dichloromethane. Petroleum ether added along with 0.2M NH4H P04, is used to remove unwanted organic matrix in the second aliquot. Both antibiotics are determined by a single gradient elution HPLC analysis. Analyte separation is achieved using LC-18-DB reversed phase analytical column. |
| Applicable Concentration Range |
Novobiocin and Virginiamycin may be detected at ≥ 500 µg kg-1 and ≥ 100 µg kg-1 concentration levels respectively, in bovine, porcine, and avian liver, muscle, or kidney tissue. The quantification levels are ≥ 1000 µg kg-1 (Novobiocin) and ≥ 200 µg kg-1 (Virginiamycin). |
| QC Requirements |
See attached SOP |
| Method Source |
USDA-FSIS |
| Method SOP |
 SOP |
| Citation |
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