Veterinary Drugs Residue Methods


 



Category Antibacterial substances, including sulfonamides and quinolones
Drug Class Name Sulfonamides
Method Title Automated Robotic Extraction, TLC Analysis for Sulfonamide Residues in Animal Tissues, Eggs, and Egg Products
Method Date 2011/05/11
Method Type Screening
Scope and Application The method is suitable for the analysis of the following sulphonamide residues: Sulfathiazole, Sulfadiazine, Sulfachloropyridazine, Sulfamethazine, Sulfamethoxazole, Sulfamethoxypyridazine, Sulfaquinoxaline, Sulfaethoxypyridazine, Sulfadimethoxine, Sulfadoxine, Sulfamerazine, Sulfisoxazole, Sulfamethizole in muscle and liver tissues of porcine, bovine, and avian species, and processed products.
Method Summary Liver and muscle samples are blended/homogenized in ethyl acetate and centrifuged. Eggs and egg products are thoroughly mixed in ethyl acetate and centrifuged as well. The extracts are then spotted onto the pre-absorbant layer of LK6D thin layer plate and developed in methanol followed by chloroform/t-butanol (80,20) after drying the plate. The plate is then dipped in fluorescamine and scanned using a densitometer (410 plus or minus 10nm excitation).
Applicable Concentration Range Sulfathiazole, Sulfadiazine, Sulfachloropyridazine, Sulfamethazine, Sulfamethoxazole, Sulfamethoxypyridazine, Sulfaquinoxaline, Sulfaethoxypyridazine, Sulfadimethoxine, Sulfadoxine, Sulfamerazine, Sulfisoxazole, Sulfamethizole may be detected at ≥ 50 µg kg-1 concentration level using the method in muscle and liver tissues of porcine, bovine, and avian species, and processed products.
QC Requirements See attached SOP
Method Source USDA-FSIS
Method SOP PDF icon SOP 
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