| Category |
Antibacterial substances, including sulfonamides and quinolones |
| Drug Class Name |
Sulfonamides |
| Method Title |
Automated Robotic Extraction – TLC Analysis for Sulfonamide Residues in Animal Tissues, Eggs, and Egg Products |
| Method Date |
2011/05/11 |
| Method Type |
Screening |
| Scope and Application |
The method is suitable for the analysis of the following sulphonamide residues: Sulfathiazole, Sulfadiazine, Sulfachloropyridazine, Sulfamethazine, Sulfamethoxazole, Sulfamethoxypyridazine, Sulfaquinoxaline, Sulfaethoxypyridazine, Sulfadimethoxine, Sulfadoxine, Sulfamerazine, Sulfisoxazole, Sulfamethizole in muscle and liver tissues of porcine, bovine, and avian species, and processed products. |
| Method Summary |
Liver and muscle samples are blended/homogenized in ethyl acetate and centrifuged. Eggs and egg products are thoroughly mixed in ethyl acetate and centrifuged as well. The extracts are then spotted onto the pre-absorbant layer of LK6D thin layer plate and developed in methanol followed by chloroform/t-butanol (80:20) after drying the plate. The plate is then dipped in fluorescamine and scanned using a densitometer (410 ± 10nm excitation). |
| Applicable Concentration Range |
Sulfathiazole, Sulfadiazine, Sulfachloropyridazine, Sulfamethazine, Sulfamethoxazole, Sulfamethoxypyridazine, Sulfaquinoxaline, Sulfaethoxypyridazine, Sulfadimethoxine, Sulfadoxine, Sulfamerazine, Sulfisoxazole, Sulfamethizole may be detected at ≥ 50 µg kg-1 concentration level using the method in muscle and liver tissues of porcine, bovine, and avian species, and processed products. |
| QC Requirements |
See attached SOP |
| Method Source |
USDA-FSIS |
| Method SOP |
 SOP |
| Citation |
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