| Category |
Antibacterial substances, including sulfonamides and quinolones |
| Drug Class Name |
Tetracyclin, Chlortetracycline and Oxytetracycline |
| Method Title |
Qualitative Identification of Tetracyclines in Tissues |
| Method Date |
2011/09/19 |
| Method Type |
Screening, Quantitative |
| Scope and Application |
The method is suitable for the identification of Chlortetracycline hydrochloride, Oxytetracycline hydrochloride and Tetracycline hydrochloride in bovine, porcine, ovine muscle, kidney and liver as well as poultry muscle and kidney. |
| Method Summary |
Fresh or partially thawed samples carefully dissected to avoid fat and connective are finely minced or ground and extracted with Mcllvaine buffer/EDTA solution by centrifugation. The extracts are then filtered through a GFB filter paper and cleaned-up by passing through C18 SPE catridges. The tetracyclines are eluted from the cartridge using methanolic oxalic acid, evaporated and reconstituted with aqueous methanol prior to analysis using a DIONEX Ultimate 3000 UHPLC connected to a VWD-3400 detector. A Syncronis C8 analytical column was used for separation. |
| Applicable Concentration Range |
Using this method, Chlortetracycline hydrochloride, Oxytetracycline hydrochloride and Tetracycline hydrochloride may be quantified at ≥ 500 µg kg -1 concentration level in bovine, porcine, ovine muscle, kidney and liver as well as poultry muscle and kidney |
| QC Requirements |
|
| Method Source |
USDA-FSIS |
| Method SOP |
 SOP |
| Citation |
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