| Category |
Steroids |
| Drug Class Name |
Trenbolone: An anabolic steroid |
| Method Title |
Determination of Trenbolone by ELISA |
| Method Date |
2004/11/30 |
| Method Type |
Screening |
| Scope and Application |
This method is suitable for the qualitative analysis of trenbolone in bovine liver. |
| Method Summary |
Liver/muscle samples homogenized in 0.5 M sodium acetate buffer (pH 4.8) are hydrolysed using glucuronidase/arylsulfatase followed by repeated centrifugation in tert-butylmethylether. The supernatant is evaporated to dryness and the residue reconstituted in methanol prior to cleanup on a RIDA® C18 column. After a series of cleanup steps the analyte is eluted using 80% methanol and then diluted with deionized water. Aliquots are then analysed on ELISA plates with absorbance measured at 450 nm against an air blank. |
| Applicable Concentration Range |
Trenbolone may be detected in bovine liver at the concentration level ≥ 5 µg kg -1. |
| QC Requirements |
See attached SOP |
| Method Source |
USDA-FSIS |
| Method SOP |
 SOP |
| Citation |
|