| Category |
Steroids |
| Drug Class Name |
Anabolic Steroid |
| Method Title |
Confirmation of a and ß-Trenbolone by GC/MS/MS |
| Method Date |
2005/01/03 |
| Method Type |
Confirmatory, Quantitative |
| Scope and Application |
This method can be used to confirm the presence of α-trenbolone and β-trenbolone in bovine liver and muscle, respectively. |
| Method Summary |
Homogenized liver/muscle tissue in 0.04 M sodium acetate buffer is digested with β glucuronidase and extracted in acetonitrile by centrifugation. The supernatant is mixed with sodium hydroxide, hexane and dichloromethane prior to shaking and subsequent centrifugation. Collect the middle layer (after repeated extraction) and mix with water. Centrifuge again and then discard the bottom aqueous layer. Evaporate remaining solution to dryness and dissolve the residue in water before cleaning up using C18 cartridges. The analyte is subsequently eluted with 80:20 methanol:water from the cartridge. The eluate is dissolved in toluene, centrifuged and stored at -10º C overnight before further cleanup using silica cartridges. here the analyte is eluted with 20:80 acetone:toluene. This is dried and the residue dissolved in ethyl acetate and then mixed with BSTFA/TMSI derivatization agent prior to GCMSMS (Ion-trap) analysis. A GC analytical column – DB-5 ms, 30m, 0.25 mm i.d., 0.25 μm film thickness is used. |
| Applicable Concentration Range |
Alpha-trenbolone and β-trenbolone may be detected in bovine liver and muscle at the concentration level of ≥ 5 µg kg -1. |
| QC Requirements |
See attached SOP |
| Method Source |
USDA-FSIS |
| Method SOP |
 SOP |
| Citation |
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