| Category |
Antibacterial substances, including sulfonamides and quinolones |
| Drug Class Name |
Sulfonamides |
| Method Title |
Determination of Sulfonamides in liver. PR-RES-22_Determinacion_de_sulfas_en_higado rev 2 |
| Method Date |
2009/01/01 |
| Method Type |
Screening, Confirmatory, Quantitative |
| Scope and Application |
This technique is useful for the detection/quantification of residues of the following sulfonamides in liver of cattle, sheep, horses and pigs: sulfacetamide, sulfadiazine, sulfathiazole, sulfamerazine, sulfamethoxipyridazine, sulfamethazine, sulfaclorpyridazine, sulfamethoxazole, sulfaquinoxaline, sulfadimethoxine. |
| Method Summary |
The sulfonamides are extracted from the samples by centrifugation using acetic acid /acetone and ethyl acetate. The supernatant is cleaned-up using Varian Bond Elut – NH2 and SCX SPE columns. Methanol is eluted from the NH2 columns and the eluate loaded onto SCX column from which the sulfas are eluted using a mixture of methanol: ammonium hydroxide. This is evaporated to dryness with nitrogen in a water bath and the residue dissolved in acetone. The acetone is also evaporated and the final residue dissolved in the mobile phase (acetonitrile:acetate buffer) prior to analysis using HPLC/DAD connected to a LiChrosorb RP-C18 analytical column. |
| Applicable Concentration Range |
Sulfacetamide, sulfadiazine, sulfathiazole, sulfamerazine, sulfamethoxipyridazina, sulfamethazine, sulfaclorpyridazina, sulfamethoxazole, sulfaquinoxaline, sulfadimethoxine may be detected in liver of cattle, sheep, horses and pigs at ≥ 50 µg kg-1 concentration level. |
| QC Requirements |
See attached SOP |
| Method Source |
The Dr. Miguel C. Rubino Laboratory, Veterinary Laboratories Division (DILAVE), Ministry of Agriculture, Livestock and Fisheries, Republic of Uruguay |
| Method SOP |
 SOP |
| Citation |
Citation |
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