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 Journal title : Journal of Applied Biological Chemistry Volume 57, Issue ,2,  pp.141-144. Publisher : The Korean Society for Applied Biological Chemisty. DOI : 10.3839/jabc.2014.021
The effects of electron beam irradiation on control of Myzus persicae and Planocococcus citri (Risso) were evaluated with the changes of mortality, emergence rate, fecundity, and egg hatchability. As a result of this study, M. persicae and P. citri (Risso) was not directly affected by electron beam irradiation with low doses (30, 60, 90, and 120 Gy) but the irradiation had effects on inhibition of development and reproduction of the pests.
Lee, Gyeong-Ae  ;  Park, Min-Goo  ;  Cho, Jae-Young ;
electron beam . insect/pest control . irradiation . Myzus persicae . Planococcus citri (Risso)
 Korean journal of applied entomology Volume 53, Issue ,4, pp.391-398. Publisher : Korean Society of Applied Entomology
 DOI : 10.5656/KSAE.2014.10.0.054
This study investigated inhibitory doses of electron beam and X-ray irradiation by comparing their effects on the development and reproduction of four insect pests (Myzus persicae, Tetranychus urticae, Liriomyza trifolii, and Frankliniella intonsa). When M. persicae nymphs were irradiated with 100 Gy of electron beam and 30 Gy of X-ray beam, offspring production by adults that developed from the treated nymphs was completely inhibited. When M. persicae adults were irradiated with 200 Gy of electron beam and 50 Gy of X-ray beam, emergence of the  generation was inhibited. However, these two ionizing radiations did not affect adult longevity. When T. urticae eggs were irradiated with 150 Gy of electron beam and 50 Gy of X-ray beam, egg hatching was completely inhibited. When L. trifolii pupae were irradiated, the emergence rate decreased with increasing doses of X-ray irradiation. After F. intonsa adults were irradiated with 250 Gy of electron beam and 200 Gy of X-ray beam, egg hatching of the  generation was completely suppressed.
Yun, Seung-Hwan  ;  Kim, Minjun  ;  Kim, Hyunah  ;  Lee, Seon-Woo  ;  Yoo, Dae Hyun  ;  Kim, Hyun Kyung  ;  Koo, Hyun-Na  ;  Kim, Gil-Hah ;
Insect pests . Electron beam . X-ray . Inhibition dose
J. Econ. Entomol. 108(3): 868–872; DOI: 10.1093/jee/tov068
Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5–7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by >99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1–4%) caused a high level of sterility (>99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread.
Aethina tumida, honey bee, Apis mellifera, x-ray radiation, sterile insect technique
Tissue and Cell; 46(4):274-285. DOI:10.1016/j.tice.2014.06.003 ·
In the present study we describe the morphology of the male reproductive apparatus and sperm ultrastructure of the red palm weevil - an invasive pest of several palm tree species - as well as the most important steps of spermatogenesis. The reproductive apparatus consists of a pair of testes (each formed by two lobes) a long tube-like accessory gland, a prostate gland and a small accessory gland. Characteristic features of the sperm are: 90 to 100 μm total length, 10 μm nucleus, two mitochondrial derivatives, two accessory bodies, one well-developed puff-like structure and a typical insect 9 + 9 + 2 flagellar axoneme. One of the methods used for the biological control of pests is the Sterile Insect Technique (SIT), for the evaluation of which we make a preliminary comparison of the sperm ultrastructure of non-irradiated and irradiated weevils (at a dose of 80 Gray). Feasibility of SIT to control red palm weevil (Rhynchophorus ferrugineus Olivier): An integrated physiological, ecological and genetic approach. Available from: [accessed Jul 8, 2015].
Paoli Francesco,Dallai Romano, Cristofaro Massimo, Arnone Silvia, Francardi Valeria, Roversi Pio Federico
Genital apparatus; Genital system; Insect spermatogenesis; Sterile insect technique (SIT); TEM
Atti Accad Naz Ital Entomol Rendiconti Anno LXI:239–246
The red palm weevil (RPW), Rhynchophorus ferrugineus (Oliver) (Coleoptera: Dryophthoridae), a weevil native to South- eastern Asia and Melanesia where it is a serious pest of coconuts, is considered the most dangerous pest of Canarian palm trees and date palm trees in Southern Europe, Northern Africa and Middle Asia. Aim of this work was to evaluate the feasibility to control the weevil pest by implementation of sterile insect technique (SIT) , particularly useful in area-wide IPM programs. Bioassays carried out at the ENEA C. R. Casaccia facilities were addressed to test the effects of different doses of γ rays on the weevil reproductive physiology and mating behavior. In spite of the apparent drawbacks in reproductive behavior of females observed in field (polyandry and high levels of fertility), laboratory experiment s satisfy some important requisites for the application of this technique (last male sperm precedence, high vitality and ability of mating of irradiated males) and suggest the possibility to use SIT in particular geographical contexts. Results and perspectiv es are discussed
Musmeci S, Cristofaro M, Arnone S, Sasso R, Baccaro S, Pasquali A, Catarci S
Rhynchophorus ferrugineus, Sterile Insect Technique, red palm weevil, integrated pest management
ESA 60TH Annual Meeting, Knoxville, Tennessee (USA)
Cristofaro M, Arnone S, Musmeci S, Sasso R, Lai A, De Biase A, La Marca A, Belvedere S, Marcari V, Senia G, Catarci S
Bull. Entomol. Res. 103:241–250
Rhynchophorus ferrugineus (Coleoptera, Curculionidae) is the most threatening pest of palms worldwide. The potential of gamma-irradiated males to spread a pathogenic strain of the entomopathogenic fungus Beauveria bassiana (Ascomycota: Clavicipitaceae) to control this pest was studied. First, the effects of gamma irradiation (15 and 25 Gy) on the mating success and performance of adult males irradiated at age one day were studied in the laboratory. Although male longevity decreased after irradiation (118.6 vs. 244.7 days for irradiated and control males, respectively) and their testes suffered from the treatment, fecundity of mated females did not depend on the irradiation status of the male (86.8±5.5 eggs in 15 days). However, egg hatching was significantly lower in couples with irradiated males (31.4% vs. 86.5% for irradiated and control couples, respectively), and this value decreased after a second mating (6.1% vs. 85.9%). Therefore, irradiation did not affect male sexual competiveness but sperm quality. Second, a semi-field assay was carried out to evaluate infestation in young Phoenix canariensis caused by different combinations of couples with irradiated and/or B. bassiana -challenged males. The number of immature stages found in infested palms was significantly higher when females mated with untreated males and lower when mated with irradiated males (either B. bassiana -infected or not). Some females from the fungus-challenged treatments showed post-mortem hyphal growth, and this horizontal transmission proves that irradiated males could act as a vector for B. bassiana and should be considered as a new method to improve the biological control of R. ferrugineus.
Llácer E, Santiago-Álvarez C, Jacas JA
gamma irradiation, horizontal transmission, entomopathogenic fungus, palm pest, Phoenix canariensis , autodissemination, sterile males
International Journal of Research in Agricultural Sciences (IJRAS) Volume 2, Issue 1, pp. 29-33
The objective of this research was to evaluate the effect of gamma irradiation on fruit fly (Ceratitiscapitata) eggs and larvae (1st, 2nd and 3rd instars) in ‘Valencia’ oranges, and evaluate the effect of the irradiation on the chemical composition of the fruits. The fruits were artificially infested with the immature stages of the fruit fly and treated with 0, 10, 20, 30, 40, 50, 100, 150 and 200 Gy Cobalt-60 doses. The treatment with gamma irradiation can be recommended for quarantine treatment of all immature stages of C. capitata in ‘Valencia’ oranges if applied at the doses of 72.88 Gy. Larvae of 3rd instar are more radio resistent when compared to eggs and larval of 1st and 2nd instar. The doses of gamma radiation used do not affect the chemical proprieties of ‘Valencia’ orange fruits. IDIDAS COMMENT: infestation was artificial, so results may not reflect the natural situation for which a treatment must be efficacious. Also, no data on effect of the treatment (dose not stated) on fruit were given other than the comment that quality was similar to non-irradiated ‘Valencia’ orange.
Bortoli, Sergio A. De; de Albergaria, Nuno M. M. S.; Dória, Háyda O. S.; Vacari, Alessandra M.; Duarte, Rogério T.; Arthur, Valter
Ionizing Radiation, Quarantine Treatment, Disinfestation, Ceratitis Capitata, Citrus Sinensis
Pest Manag Sci. article published online: 10 September 2014
( DOI 10.1002/ps.3905
BACKGROUND: The number of insect eradication programmes is rising in response to globalisation. A database of arthropod and plant pathogen eradications covers 1050 incursion responses, with 928 eradication programmes on 299 pest and disease taxa in 104 countries (global eradication database METHODS: A subset of the database was assembled with 211 eradication or response programmes against 17 species of fruit flies (Tephritidae) in 31 countries, in order to investigate factors affecting the outcome. RESULTS: The failure rate for fruit fly eradication programmeswas about 7%, with 0% for Ceratitis capitata (n=85 programmes) and 0% for two Anastrepha species (n = 12 programmes), but 12% for 13 Bactrocera species (n = 108 programmes). A number of intended eradication programmesagainst long-established populationswere not initiated because of cost and other considerations, or evolved during the planning phase into suppression programmes. Cost was dependent on area, ranged from $US 0.1 million to $US 240 million and averaged about $US 12 million (normalised to $US in 2012). In addition to the routine use of surveillance networks, quarantine and fruit destruction, the key tactics used in eradication programmes were male annihilation, protein bait sprays (which can attract both sexes), fruit destruction and the sterile insect technique. CONCLUSIONS: Eradication success generally required the combination of several tactics applied on an area-wide basis. Because the likelihood of eradication declines with an increase in the area infested, it pays to invest in effective surveillance networks that allowearly detection and delimitationwhile invading populations are small, thereby greatly favouring eradication success. © 2014 Society of Chemical Industry
Suckling David Maxwell, Kean John M, Stringer Lloyd D,
Cáceres-Barrios Carlos, Hendrichs Jorge, Reyes-Flores Jesus
and Dominiak Bernard C.
Tephritidae; male annihilation; sterile insect technique; bait; trap; surveillance; incursions; quarantine
Biocontrol Science and Technology, 25:9, 1092-1103, DOI: 10.1080/09583157.2015.1030723
Diachasmimorpha longicaudata is a koinobiont larval parasitoid that is currently used to control fruit flies of the genera Anastrepha, Ceratitis and Bactrocera. In the rearing process, a fraction of the host larvae that are exposed to parasitoids escape from parasitism and develop into viable and fertile flies. This creates the need to eliminate emerging flies before the parasitoids are shipped for release, increasing costs due to additional handling steps. Exposure of fly eggs or larvae to gamma-irradiation before they are parasitised has been used to reproductively sterilise hosts, or even inhibit their emergence. Our aim was to determine whether X-ray radiation applied to Anastrepha fraterculus third instar larvae before they are exposed to parasitoids, inhibits fly emergence in non-parasitised larvae without affecting the performance of the parasitoids that emerge from parasitised larvae. Three X-ray doses: 6250.2 R, 8333.6 R and 10417 R (equivalent to 60, 80 and 100 Gy, respectively) and one γ-ray dose (100 Gy) were tested. Fly emergence decreased with increasing doses of radiation, showing null values for the higher X-ray dose and the dose of 100 Gy. Irradiation showed either no impact or a positive effect on parasitism rate and fecundity. Sex rate was biased towards females in almost every dose. We conclude that the two types of radiation evaluated here were equally effective in suppressing fly emergence with no detrimental effects on the biological quality of the produced parasitoids. X-rays offer an alternative method of irradiation than the conventional radiation source, i.e. γ-rays. These results represent a significant improvement in the development of a biological control programme against A. fraterculus.
Bachmann Guillermo E., Paladino Leonela Z. Carabajal, Claudia A. Conte, Francisco Devescovi, Fabián H. Milla, Jorge L. Cladera, Diego F. Segura & Mariana M. Viscarret
biological control; gamma rays; Anastrepha fraterculus; Diachasmimorpha longicaudata; fruit fly pests; natural enemies
J. Rediat. Res. Appl. Sci. 7:110-115.
Three substerilizing doses 50, 100 and 150 Gy of gamma radiation were tested against full grown male and female pupae or against full-grown male or female pupae of Agrotis ipsilon. The results showed that fecundity of irradiated females crossed with irradiated males was decreased by increasing irradiation dose. The decrease in egg hatchability % and increase in sterility % induced by gamma radiation were found to be positively correlated with the dose level. The parentage of larval and pupal mortality increased significantly (p
Salem HM, Fouda MA, Abas A A, Ali WM, Gabarty A ().
Gamma irradiation Substerilizing doses Agrotis ipsilon
Journal of Entomology and Nematology, Vol.7(3), pp. 26-29. DOI: 10.5897/JEN2015.0124
Fruit flies are regarded as one of the most devastating pest of fruits and vegetables on earth planet. Generally chemical control is implemented for their control but it poses lot of eco-environmental concerns so the emphasis is now turning towards eco-friendly management practices. Bio-control is an efficient and environmentally sound approach and augmentation is primarily focus on classical biological control program. In this study, eight sub-sterilizing doses of 0, 20, 30, 40, 50, 60, 70 and 80 were tested against Bactrocera zonata and Bactrocera cucurbitae pupae. The results showed that radiation prolong the duration of pupal stage and hatching is reduced by applying radiation. This also shows that when the quantity of the radiation increases, the adult emergence decreases. This study could be very useful in exploiting the potential host for longer period of time for culturing their pupal parasitoids.
Sub sterilizing doses, radiation, fruit flies and emergence.
International Journal of Applied Radiation and Isotopes 20: 791-795. doi:10.1016/0020-708X(69)90042-8
The effect of gamma radiation from a cobalt-60 Source on adults of the fruit fly (Dacus ciliatus Loew) following pupal exposure was determined. When 7 days old pupae were irradiated at 5 krad., partial sterility with normal longevity was observed in the adults while irradiation at doses of 8·5 and 10 krad. induced complete sterility with normal longevity and preoviposition period which lasted throughout their adult life. The optimum sterilizing dose for this fly thus lies in the range of 8·5–10 krad. and can be safely utilized for the eradication of this fly from Karachi area by sterile male release method.
Huque H. and Ahmad C.R.
Dacus tephritidae sterility radiation
Journal of Northwest A&F University 40: 57-61
The experiment was to study elimination effects of irradiation against different stages citrus red mite (Panonychus citri Mcgregor) and to conform the effective dosage in order to achieve inspection and quarantine. The eggs, larvae, protonymphs of citrus red mite were selected and irradiated by gamma irradiation (0, 100, 200, 300, 400 Gy), while adults were at the dosage of 0, 200, 400, 600 and 800 Gy). The effect of irradiation against hatching rates and mortality of different stages of citrus red mites was studied. It was found that after being irradiated at the dosage of 200 Gy, the 24 h-old eggs of citrus red mites were not hatched, so the gamma irradiation at the dosage of 200 Gy obviously had lethal effect on the eggs. After irradiated at the dosage of 400 Gy, the mortality of the larvae was as high as 96%. Gamma irradiation among the 300-400 Gy made the larvae sterile and the dosage of 400 Gy was sterile dosage to the protonymphs of citrus red mite. The adults laid less eggs which did not hatch at more than dosage of 400 Gy. After 15 days, the mortality of adult mites irradiated at the dosage of 400-600 Gy was 100%; while irradiated at the dosage of 800 Gy, the mortality was 100% after 13 days. So the irradiation among the 400-800 Gy made the adults sterile or lethal. The gamma irradiation at the dosage 400 Gy made citrus red mite at different stages lethal or sterile. So the citrus fruits irradiated at 400 Gy could achieve quarantine treatment requirement.
Zhu FW, Deng YY, Weng QF, Hu M.Y.
mites, Panonychus citri, plant protection, irradiation, Citrus
Radiation Physics and Chemistry 90: 111-119.
Osouli Sh, Ziaie F, Haddad Irani Nejad K, Moghaddam M.
Acta Agriculturae Zhejiangensis 25:533-536.
Wu Q, Lin WC, Wang BK, Qi WY, Xiong LD, Wei JY, Chen JY.
mite, China
Journal of Huazhong Agricultural University 21: 347-351.
Zhou LJ, Hu MY, Huang JG, Xu WS, Cheng DM, Wang WX.
citrus rust mite, China
Nukleonika 41: 81-88.
Ignatowicz S, Wróblicka-Sysiak M.
bulb mite
Florida Entomologist
Arthur V, Machi AR.
Acari, Eriophyidae
Final Report to International Atomic Energy Agency.
Ignatowicz S.
Aculus schlechtendali, Eriophyes pyri
Florida Entomologist
Arthur V, Machi AR.
Acari, oxygen, air, atmospher, mites
Entomological Research, 45: 110–115. doi: 10.1111/1748-5967.12101
Ionizing radiation is increasingly used as an alternative to post-harvest crop fumigation by methyl bromide. We studied the effects of gamma irradiation on Helicoverpa assulta (Lepidoptera: Noctuidae) at different stages of development to determine the minimal dose for the prevention of normal emergence of adults. We selected five doses of gamma rays (100, 200, 300, 400 and 500 Gy) based on preliminary experiments and irradiated eggs, larvae, pupae and adults. A dose of 100 Gy to eggs allowed 21.83% of larvae to pupate, but these all died during the pupal stage. A dose of 100 Gy to last-instar larvae caused larval or pupal death, or the emergence of abnormal adults; no normal adults developed. Irradiation of pupae with doses of 300 Gy and above resulted either in their death or emergence of abnormal adults; however, after 100 or 200 Gy, normal adults emerged and F1 eggs were produced, but no eggs hatched. Following irradiation of adults, eggs were produced at all doses, although the numbers were significantly decreased compared to untreated controls (P < 0.05; 69.45–125.50 vs. 475.05 eggs per female); however, none of the eggs hatched. As prevention of normal emergence is a key outcome for measuring the effectiveness of radiation, then the 100 Gy dose was effective for irradiation of eggs and larvae, and 300 Gy for pupae.
Park, J. S., Lee, J. Y., Jeong, S. Y., Ahn, S.-J. and Kim, I.
Gamma irradiation; Helicoverpa assulta; Noctuidae; Oriental tobacco budworm; quarantine
Journal of Insect Physiology Volume 75, Pages 85–90
• Argiope keyserlingi males perform vibratory courtship before mating with females.
• The thread assay is an appropriate proxy representing courtship on a female web.
• We quantify the dosage of gamma ray resulting in complete sterilization.
• The correct dosage of irradiation does not affect male courtship behaviour.
The sterile male technique is a common method to assign paternity, widely adopted due to its relative simplicity and low cost. Male sterility is induced by exposure to sub lethal doses of chemosterilants or irradiation, the dosage of which has to be calibrated for every species to provide successful male sterilisation, without affecting male physiology and behaviour. While the physiological effects of sterilisation are usually assessed for each study, the behavioural ones are rarely analysed in detail. Using the orb web spider Argiope keyserlingi as a model we first tested (1) the validity of the thread assay, which simulates male courtship behaviour in a standardised context, as a proxy representing courtship on a female web. We then investigated (2) the effectiveness of male sterilisation via irradiation and (3) its consequences on male courtship behaviour. Our results validate the thread assay and the sterile male technique as legitimate tools for the study of male courtship behaviour and fertilisation success. We show that these techniques are time and cost effective and reduce undesirable variation, thereby creating opportunities to study and understand the mechanisms underlying sexual selection.

Magris Martina, Wignall Anne E., Herberstein Marie E.
Reproductive behaviour;
Argiope keyserlingi
Emomologia Experimentalis et Applicata 155: 117-122 DOl: 10.1 111/eca. l2289
The Ethiopian fruit fly, Dacus ciliatus (Loew) (Diptera: Tephritidae), is a significant pest of cucurbit crops in Asia and Africa and is currently controlled with insecticides. The sterilizing effect of gamma radiation on D. ciliatus adults was investigated to assess the suitability of sterile insect technique (SIT) for use as an alternative, non-chemical strategy for the control of this pest. Late pupae (48 h before emergence) were irradiated with 60, 80, 100, 120, and 140 Gy of gamma rays emitted by a 60Co source. Following emergence, the biological characteristics of the experimental cohorts (including all possible male-female combinations of irradiated and untreated flies) were recorded. No significant negative effects of irradiation on pupal eclosion or the ability of newly emerged flies to fly were observed. Samples of eggs at reproductive fly-ages (12-, 15-, and 17-day-old pairs) were collected and their hatch rates were assessed. At 60 Gy, females were completely sterilized, whereas complete sterilization of the males was observed only at 140 Gy (a small amount of fertility persisted even at 120 Gy). In addition to the above experiments, three fruit infestation trials were conducted with zucchini [Cucurbita pepo L. (Cucurbitaceae)] as the plant host and the pupae produced in those trials were collected and recorded. We observed significant (ca. 10%) infestation following treatment with up to 120 Gy and zero progeny only at 140 Gy, mirroring the egg-hatch results. Our findings support the feasibility of SIT for the control of D. ciliatus.
Rempoulakis, P., Castro, R., Nemny-Lavy, E. and Nestel, D.
sterile insect technique;
J. Econ. Entomol. 108(1): 88–94; DOI: 10.1093/jee/tou013
The fruit fly Bactrocera tau (Walker) is an important quarantine pest that damages fruits and vegetables throughout Asian regions. Host commodities shipped from infested areas should undergo phytosanitary measures to reduce the risk of shipping viable flies. The dose–response tests with 1-d-old eggs and 3-, 5-, 7-, 8-d-old larvae were initiated to determine the most resistant stages in fruits, and the minimum dose for 99.9968% prevention of adult eclosion at 95% confidence level was validated in the confirmatory tests. The results showed that 1) the pupariation rate was not affected by gamma radiation
except for eggs and first instars, while the percent of eclosion was reduced significantly in all instars at all radiation dose; 2) the tolerance to radiation increased with increasing age and developmental stage; 3) the estimated dose to 99.9968% preventing adult eclosion from late third instars was 70.9 Gy (95% CL: 65.6–78.2, probit model) and 71.8 Gy (95% CL: 63.0–87.3, logit model); and iv) in total, 107,135 late
third instars cage infested in pumpkin fruits were irradiated at the target dose of 70 Gy (62.5–85.0, Gy measured), which resulted in no adult emergence in the two confirmatory tests. Therefore, a minimum dose of 85 and 72 Gy, which could prevent adult emergence at the efficacy of 99.9972 and 99.9938% at the 95% confidence level, respectively, can be recommended as a minimum dose for phytosanitary treatment
of B. tau in any host fruits and vegetables under ambient atmospheres.
Zhan Guoping , Ren Lili , Shao Ying , Wang Qiaoling , Yu Daojian , Wang Yuejin , Li Tianxiu
Bactrocera tau, phytosanitary irradiation, gamma radiation, irradiation, pumpkin
Journal of Economic Entomology 107: 154-160.                   DOI: 10.1603/EC13226
Phlyctinus callosus (Boheman) (Coleoptera: Curculionidae) is a pest of major phytosanitary concern for some of South Africa's biggest export markets such as the United States and Europe because this pest does not occur there. At present, fumigation with methyl bromide is the only postharvest disinfestation treatment against this pest; therefore, sustainable alternatives are needed. One such alternative is irradiation treatment of whole pallets of packed fruit to sterilize insects that may be present within the cartons. Wild adult P. callosus weevils were treated with 5, 10, 20, 40, and 80 Gy of gamma-irradiation and then cross mated to breed with either treated or nontreated adults of the opposite sex. Fecundity and fertility were monitored and recorded. Trials were conducted during the 2009-2010 and 2010-2011 fruit harvesting seasons. The results from both seasons indicated that irradiation did not affect fecundity but fertility was significantly affected, decreasing as irradiation doses increased. Egg hatch was zero for mating crosses that involved females weevils treated with a dose of 80 Gy gamma-irradiation. Probit analysis indicated that in the first season, the estimated LD95 for crosses involving treated males and treated females was 30 Gy, while in the second season it was 49.5 Gy. Respective estimated LD99S were 47.9 and 169.4 Gy. Ultimately, a dose lower than the current generic dose of 400 Gy, approved for irradiation disinfestation treatments, would control P. callosus should they occur in packed export fruit.
Duvenhage AJ, Johnson SA.
Phlyctinus callosus
Journal of Stored Products Research 59:108-112
The biological activity of basil (Ocimum basilicum L.) oil vapor was tested against the stored product pest rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae). Adult weevils were exposed to seven different concentrations of basil oil ranging from 0.12 ml/mle0.60 ml/ml in Petri dishes and mortality was assessed at 3, 4 and 5 d post treatment. Mortality increased with increasing exposure time and basil oïl concentration. At 3, 4 and 5 d post treatment, the LC50 values were 8.14, 6.50 and 4.91 ml/ml of basil oil, respectively. S. oryzae was also exposed to 0.12 or 0.24 ml/ml basil oil and irradiated at 20, 40, 60, 80, 100 or 120 Gy in Petri dishes before mortality assessment at 5 d post treatment. The effectiveness of irradiation treatment against S. oryzae was enhanced by exposure to basil oil. S. oryzae exposed to 0.12 and 0.24 ml/ml of basil oil were 4.1 and 5.3 times more sensitive to irradiation, respectively, compared to control weevils treated only with irradiation. The effect of basil oil and irradiation on mortality was synergistic when used in  combination against S. oryzae in packaged rice. The type of rice package affected treatment efficacy. In paper rice packages, 78% mortality was observed with 2.5 ml/ml basil oil and a radiation dose of 200 Gy at 5 d post treatment. In plastic packages, 100% mortality was achieved with 0.83 ml/ml basil oil and a radiation dose of 200 Gy at 5 d post treatment. Basil oil has potential as a synergist to lower the radiation dose required to control phytosanitary pests.
Hossain Farah, Lacroix Monique, Salmieri Stephane, Vu Khanh, Follett Peter A.
Sitophilus oryzae
Basil oil
Ionizing radiation
Radiation sensitivity
Phytosanitary treatment
Journal of Stored Products Research 52: 63-67.
Irradiation is a quarantine treatment option for stored products pests. Dose response tests were conducted to identify a post-harvest radiation treatment that would control rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae) in rice. Rice infested with adult or immature weevils was treated at radiation doses of 30, 60, 90, or 120 Gy, or left untreated as a control. Live and dead beetles were counted weekly for 15 24 weeks. Treatment of adult weevils at a radiation dose of 120 Gy resulted in no live adults after two weeks, indicating that this radiation dose caused adult mortality and sterility, whereas a total of 1261 adult beetles emerged during 24 weeks in the untreated controls. Treatment of immature life stages (a mixture of eggs, larvae and pupae) with a radiation dose of 90 or 120 Gy resulted in no adults emerging after five or two weeks, respectively, indicating that these doses prevented reproduction, whereas a total of 4275 adults emerged throughout 15 weeks in the untreated controls. Weight loss of rice infested with immature or adult weevils was significantly reduced by irradiation treatment at 60 Gy and 120 Gy. In a large-scale confirmatory test, a radiation dose of 120 Gy applied to 38,025 adult weevils in rice resulted in no reproduction. Irradiation at 120 Gy will provide quarantine security for rice weevil, and prevent post-irradiation weight loss caused by insect feeding in the commodity. Irradiation may be particularly helpful in controlling phosphine-resistant populations, and could help manage resistance by preventing the spread of resistant weevils in exported grains. Irradiation is a control option for stored product insects. Rice weevil is the most serious pest of stored rice worldwide. Adult and immature weevils were treated at 0, 30, 60, 90 or 120 Gy in rice. Radiation treatment at 120 Gy sterilized rice weevil and prevented further damage. Irradiation can prevent the spread of phosphine-resistant weevils in exported grain.
Follett PA, Snook K, Janson A, Antonio B, Haruki A, Okamura M, Bisel J.
Sitophilus oryzae;
Rice weevil;
X-ray irradiation;
Phosphine resistance;
Journal of Economic Entomology 107: 161-165                   doi:
The mango pulp weevil, Sternochetus frigidus (F.), is an important quarantine pest preventing the export of mangoes from the Philippines to the United States and other countries. Previously, a radiation dose of 100 Gy was proposed for phytosanitary treatment of S. frigidus based on dose-response studies with larvae, pupae, and adult weevils. To validate an irradiation treatment, large-scale confirmatory tests were conducted with adults (the most radiation-tolerant stage) in mangoes at 100 and 150 Gy. After treatment, adults were removed from fruit, sexed, and mated in pairs to observe any reproduction. At 100 Gy, adults laid a small number of eggs but none of the eggs hatched. At 150 Gy (measured doses 96.7-164.1 Gy),4,559 treated weevils laid no eggs, indicating that this dose caused complete sterility. Irradiation treatment with a minimum absorbed dose of 165 Gy will therefore provide quarantine security for S. frigidus in exported Philippine mangoes.
Obra GB, Resilva SS, Follett PA, Lorenzana LRJ.
Sternochetus frigidus, mango pulp weevil, postharvest phytosanitary treatment, quarantine pest, disinfestation
Apoptosis : an International Journal on Programmed Cell Death  20(1):1-9 (DOI: 10.1007/s10495-014-1055-3).
Transcriptional activation of pro-apoptotic genes in response to cytotoxic stimuli is a conserved feature of the cell death pathway in metazoans. However, understanding the extent of this conservation in insects has been limited by the lack of known pro-apoptotic genes in non-drosophilids. Recently, we described the pro-apoptotic genes, Asrpr and Ashid, from the tephritid, Anastrepha suspensa, that now allow us to explore the conservation of pro-apoptotic gene regulation between a tephritid and drosophilids. In this study, we determined the developmental profiles of Asrpr and Ashid transcripts during embryogenesis and in embryos exposed to γ-irradiation. Transcript levels of both genes determined by qRT-PCR were low throughout embryogenesis, with strong Ashid expression occurring during early to mid-embryogenesis and Asrpr expression peaking in late embryogenesis. This correlated to acridine orange stained apoptotic cells first appearing at 17 h and increasing over time. However, when irradiated at 16 h post-oviposition embryos exhibited significant levels of apoptosis consistent with strong induction of Asrpr and Ashid transcript levels by γ-irradiation in young embryos <24 h post-oviposition. Furthermore, embryos irradiated <24 h post-oviposition failed to hatch, those irradiated between 24 and 32 h had increased hatching rates, but between 48 and 72 h irradiation had no effect on egg hatching. This indicates a transition of embryos from an irradiation-sensitive to irradiation-resistance stage between 24 and 48 h. Throughout post-embryonic development, the two pro-apoptotic genes share similar patterns of up-regulated gene expression, which correlate to ecdysone-induced developmental events, especially during metamorphosis. Together these results provide the first direct evidence for a conserved molecular mechanism of the programmed cell death pathway in insects.
Nirmala X,  Schetelig MF,  Zimowska GJ,  Zhou L,  Handler AM
Ashid, Asrpr, Irradiation, Ecdysteroids, Cell death, Tephritids
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