Pesticide Residue Methods

Category Others
Class Name Pesticide Specific Enforcement Method, Buprofezin
Method Title An Analytical Method for the Determination of Residues of Buprofezin at Estimated Tolerance Levels in Almonds, Cottonseed, Citrus (Lemons), and Grapes by Gas Chromatography Using Nitrogen Phosphorus Detection
Method Date 1997/07/21
Method Type Quantitative
Scope and Application Analysis for buprofezin residues Analysis of vegetables Can be adapted to most vegetables GC-NPD detection
Method Summary Extractable residues of buprofezin are removed from the crop matrix by blending with acetone. After rotary evaporation to the aqueous phase, the extracts are transferred to separatory funnels with 1 M hydrochloric acid. The acidic aqueous extracts are then cleaned up by partitioning with hexane. The hexane is back partitioned with additional 1 M hydrochloric acid. After the hexane partition is discarded, the combined acidic aqueous phase containing buprofezin is partitioned with dichloromethane. Thedichloromethane extract is dried through a sodium sulfate pad, rotary evaporated to dryness, and redissolved in organic solvent to await cleanup by Florisil column chromatography. Following clean-up, buprofezin is quantified by gas chromatography using nitrogen phosphorous detection.
Applicable Concentration Range Estimated LOQ: 0.05 mg/Kg
QC Requirements See Attached Method
Method Performance/Validation See Attached Method
Method Source IR-4
Method SOP PDF icon SOP 
Citation AgrEvo RAM No. BF-10-97