Pesticide Residue Methods

Category Carbamates and pyrethroids
Class Name Dithiocarbamate Pesticides
Method Title Determination of Ethylene bis-Dithiocarbamate in Fruits and Vegetables by HPLC-FL
Method Date 2003/07/03
Method Type Screening, Quantitative
Scope and Application For the analysis of Ethylene bis-Dithiocarbamate (EBDC) in fruits and vegetables with a reporting limit of 0.04 microgram per gram as zineb equivalent.
Method Summary A homogenized sample (50.0 grams) is extracted in 4N HCl and SnCl2 and then diluted in purified water before cleanup by ion exchange using resins in a BioRad Econo-Pak column. The analyte is eluted using NaHCO3 and derivatized with o-Phthaladehyde and 2-Mercaptoethanol involving overnight heating. The solution is further acidified with HCl and mixed well in methanol prior to analysis by HPLC/FL (excitation 290 nm, emission 447 nm). An ODS-1 analytical column (25 cm, 4.6 mm) is used for separation.
Applicable Concentration Range Ethylene bis-Dithiocarbamates can be detected in fruits and vegetables between 0.02 and 0.21 microgram per grams.
QC Requirements See uploaded SOP
Method Performance/Validation
Method Source Canadian Food Inspection Agency
Method SOP PDF icon SOP 
Citation Canadian Food Inspection Agency, SPR-002-V2.4