Veterinary Drugs Residue Methods


Category Compounds included in Annex IV to Council Regulation (EEC) No. 2377/90
Drug Class Name Chloramphenicol
Method Title Screening for Chloramphenicol by ELISA
Method Date 2012/07/23
Method Type Screening
Scope and Application This method is suitable for screening for the presence of chloramphenicol in poultry, beef, swine and catfish muscle.
Method Summary Homogenized tissue is extracted by centrifugation in ethyl acetate and the extract evaporated to dryness. The residue is dissolved in n-hexane before adding a sample dilution buffer followed by further centrifugation. Aliquots of the lower aqueous layer are then analysed by Competitive ELISA. The plate reader is set at 450 nm.
Applicable Concentration Range Chloramphenoicol may be detected in poultry, beef, swine and catfish muscle at the concentration level of ≥ 0.25 µg kg-1
QC Requirements See attached SOP
Method Source USDA-FSIS
Method SOP PDF icon SOP 
Citation USDA-FSIS Chemistry Laboratory Guidebook