Veterinary Drugs Residue Methods


Category Antibacterial substances, including sulfonamides and quinolones
Drug Class Name Florfenicol, a fluorinated synthetic analog of thiamphenicol
Method Title Determination and Confirmation of Florfenicol
Method Date 2010/10/12
Method Type Confirmatory, Quantitative
Scope and Application This procedure is applicable for analysis of florfenicol amine in bovine and poultry liver and muscle as well as catfish muscle.
Method Summary Bovine and poultry liver and muscle as well as catfish muscle, all free from connective tissue, are homogenised using a blender and acidified with 6N HCl (to facilitate conversion of florfenicol to florfenicol amine). After centrifugation, the homogenate pH is adjusted to 12.5 and the extract loaded onto a sorbent column. Ethyl acetate or methylene chloride (in case of substantial matrix interference) is added to the sample tubes and thoroughly mixed before loading the mixture onto the sorbent column. The eluate is evaporated to dryness and the residue dissolved in a sample buffer (10 mM KH2PO4 (pH 4.0), one percent Acetonitrile) and filtered through a 0.2 micrometer Acrodisc filter prior to analysis by reverse phase high performance liquid chromatography (HPLC) with UV detection. A C8 MAC MOD Zorbax RX analytical column is used.
Applicable Concentration Range Florfenicol amine may be analysed at the following concentrations ≥ 0.3 ppm (in bovine liver), ≥ 0.2 µg kg-1 (in bovine muscle), ≥ 1.5 µg kg-1 (in poultry liver), ≥ 0.6 µg kg-1 (in poultry muscle), and ≥ 0.5 µg kg-1 (in catfish muscle).
QC Requirements See attached SOP
Method Source USDA-FSIS
Method SOP PDF icon SOP