Veterinary Drugs Residue Methods


Category Antibacterial substances, including sulfonamides and quinolones
Drug Class Name Aminoglycosides (AMGs)
Method Title Screening and Confirmation for Aminoglycosides by LC-MS-MS
Method Date 2012/12/12
Method Type Screening, Confirmatory, Quantitative
Scope and Application The method is applicable for screening/confirming the presence of aminoglycosides (amikacin, apramycin, dihydrostreptomycin, gentamicin, hygromycin B, kanamycin, neomycin B, spectinomycin, and streptomycin) in bovine kidney, liver and muscle.
Method Summary The aminoglycoside residues are extracted by centrifugation from bovine liver, kidney and muscle tissue (free from fat and connective tissue) using a buffer containing ammonium acetate/trichloroacetic acid (NH4OAc,EDTA,NaCl,TCA buffer). Tobramycin is used as an internal standard. The sample extracts (adjusted to pH 6.50 plus minus 0.05) are cleaned-up with a weak-cation exchange sorbent using disposable pipette extraction followed by elution with ten percent formic acid in water. The final extract is analyzed using ion-pair reverse phase UHPLC/MS/MS in positive electrospray ionization mode. A C18 waters UPLC BEH column is used for separation.
Applicable Concentration Range Amikacin, apramycin, dihydrostreptomycin, gentamicin, hygromycin B, kanamycin, neomycin B, spectinomycin may be detected in bovine kidney, muscle, and liver at various levels as detailed in the attached SOP.
QC Requirements See the attached SOP.
Method Source USDA-FSIS
Method SOP PDF icon SOP