Veterinary Drugs Residue Methods


Category Antibacterial substances, including sulfonamides and quinolones
Drug Class Name Macrolides and Lincosamides
Method Title Confirmation of Macrolide/Lincosamide Antibiotics by Ion Trap HPLC/MS/MS
Method Date 2007/07/30
Method Type Screening, Confirmatory, Quantitative
Scope and Application The method is suitable for screening/confirmation of macrolides (Erythromycin, Tylosin, Tilmicosin and Tulathromycin) and Lincosamides (Lincomycin, Clindamycin and Pirlimycin) in liver, kidney and muscle tissues of avian, porcine, and bovine origin.
Method Summary Samples are made basic (pH of 9.8 to 10.2) by adding 2M K2CO3 and extracted with ethyl acetate. Analytes are then partitioned into an acidic (phosphate) buffer and further cleaned up by extraction of the buffer solution with ethyl acetate. The buffer is then made basic and analytes are re-extracted into ethyl acetate, evaporated to dryness, redissolved in methanol/water, and analyzed by ion trap HPLC/MS/MS.
Applicable Concentration Range Lincomycin, Clindamycin, Pirlimycin, Erythromycin, Tylosin, and Tilmicosin may be confirmed at at levels ≥ 100 µgkg-1, while Tulathromycin may be confirmed at levels ≥ 1000 µgkg-1.
QC Requirements See details in the SOP attached
Method Source USDA-FSIS
Method SOP PDF icon SOP