Veterinary Drugs Residue Methods


Category Steroids
Drug Class Name Melengestrol acetate, a sythetic Progestone-like compound
Method Title Screen for Melengestrol Acetate in Fat Using ELISA
Method Date 2006/02/01
Method Type Screening
Scope and Application This method can be used to screen the presence of Melengestrol Acetate (MGA) in rendered bovine fat.
Method Summary Fat rendered at 95 to 110 degrees celcius is thoroughly mixed with petroleum ether and the contents extracted by centrifugation. The supernatant is evaporated to dryness and the residue reconstituted in methanol. The solution is frozen for 45 minutes and centrifuged prior to cleanup using a RIDA C18 column. The analyte is finally eluted from the column using 80,20 methanol, distilled water and the eluate analysed on an ELISA plate. Absorbance is read at 450 nm.
Applicable Concentration Range MGA may be detected in rendered bovine fat at the concentration levels ≥ 10 µg kg -1
QC Requirements See attached SOP
Method Source USDA-FSIS
Method SOP PDF icon SOP