Veterinary Drugs Residue Methods


Category Anticoccidials, including nitroimidazoles
Drug Class Name Narasin, Anticoccidial
Method Title Narasin in Cattle and Chicken Tissues
Method Date 1991/07/01
Method Type Screening
Scope and Application The method is suitable for screeining the presence of Narasin in bovine and chicken liver, kidney, skin and fat tissues.
Method Summary Ground/blended lean liver/kidney tissue are extracted in MeOH by centrifugation and supernatant further extracted in carbon tetrachloride. This is cleaned-up using a silica gel column and anhydrous sodium sulfate. Narasin is eluted from the column with chloroform,methanol(95:5) and evaporated to dryness and the residue redissolved in chloroform,methanol (99:1) prior to a second clean-up step using alumina column. Hexane is the added to remove fat tissue and after evaporation, the residue is dissolved in methanol before analysis by thin layer chromatography. The developing solvent was a mixture of carbon tetrachloride, benzene and ethylene glycol monomethyl ether. Ground/blended skin and fat tissue samples are redistilled in hexane and centrifuged. Supernate is extracted in acetonitrile and cleanedup as above. After evaporating the hexane layer, the residue is dissolve in methanol and analysed as above.
Applicable Concentration Range As low as 5 µg kg-1 narasin standard may be spotted (and viewed) on the plate.
QC Requirements See attached SOP
Method Source USDA-FSIS
Method SOP PDF icon SOP