Veterinary Drugs Residue Methods


Category Steroids
Drug Class Name Trenbolone, an anabolic steroid
Method Title Determination of Trenbolone by ELISA
Method Date 2004/11/30
Method Type Screening
Scope and Application This method is suitable for the qualitative analysis of trenbolone in bovine liver.
Method Summary Liver/muscle samples homogenized in 0.5 M sodium acetate buffer (pH 4.8) are hydrolysed using glucuronidase/arylsulfatase followed by repeated centrifugation in tert-butylmethylether. The supernatant is evaporated to dryness and the residue reconstituted in methanol prior to cleanup on a RIDA C18 column. After a series of cleanup steps the analyte is eluted using 80 percent methanol and then diluted with deionized water. Aliquots are then analysed on ELISA plates with absorbance measured at 450 nm against an air blank.
Applicable Concentration Range Trenbolone may be detected in bovine liver at the concentration level ≥ 5 µg kg -1.
QC Requirements See attached SOP
Method Source USDA-FSIS
Method SOP PDF icon SOP