Veterinary Drugs Residue Methods


Category Antibacterial substances, including sulfonamides and quinolones
Drug Class Name Sulfonamides
Method Title Analysis of Sulfonamides in Chicken Muscle and Porcine Kidney Using HPLC UV-VIS
Method Date 2008/06/01
Method Type Screening, Quantitative
Scope and Application This method is suitable for analysis of the Sulphadiazine, Sulphathiazole, Sulphapyridine, Sulphamerazine, Sulphamethazine, Sulphamethizole, Sulphamethoxypyridazine residues in meat sample.
Method Summary Mince tissue mixed with HCl, Na2SO4 (except chicken muscle) and ethyl acetate is extracted by centrifugation after homogenization with an Ultra-Turrax. Aliquots of the supernatant are evaporated to dryness and the residue dissolved in methanol, glacial acetic acid, water and hexane prior to further centrifugation. The aqueous layer is then analysed by HPLC-UV-Vis following post-column derivation using p-dimethylaminobenzaldehyde (after eluting the sulfonamides from the analytical column, the analytes are passed through a PTFE reaction coil where derivatisation occurs). The deriavatized analytes are detected at 450 nm. A Phenomenex C-18 Luna analytical column is used for separation.
Applicable Concentration Range The residues of Sulphadiazine, Sulphathiazole, Sulphapyridine, Sulphamerazine, Sulphamethazine, Sulphamethizole and Sulphamethoxypyridazine may be detected and quantified in chicken meat and porcine kidney samples at the concentration level ≥ 50 µg kg -1.
QC Requirements See attached SOP
Method Source IAEA/FAO-Food and Environmental Protection
Method SOP PDF icon SOP