Veterinary Drugs Residue Methods


Category Antibacterial substances, including sulfonamides and quinolones
Drug Class Name Sulfonamides
Method Title Determination of Sulfonamides in liver. PR-RES-22_Determinacion_de_sulfas_en_higado rev 2
Method Date 2009/01/01
Method Type Screening, Confirmatory, Quantitative
Scope and Application This technique is useful for the detection/quantification of residues of the following sulfonamides in liver of cattle, sheep, horses and pigs: sulfacetamide, sulfadiazine, sulfathiazole, sulfamerazine, sulfamethoxipyridazine, sulfamethazine, sulfaclorpyridazine, sulfamethoxazole, sulfaquinoxaline, sulfadimethoxine.
Method Summary The sulfonamides are extracted from the samples by centrifugation using acetic acid /acetone and ethyl acetate. The supernatant is cleaned-up using Varian Bond Elut – NH2 and SCX SPE columns. Methanol is eluted from the NH2 columns and the eluate loaded onto SCX column from which the sulfas are eluted using a mixture of methanol: ammonium hydroxide. This is evaporated to dryness with nitrogen in a water bath and the residue dissolved in acetone. The acetone is also evaporated and the final residue dissolved in the mobile phase (acetonitrile:acetate buffer) prior to analysis using HPLC/DAD connected to a LiChrosorb RP-C18 analytical column.
Applicable Concentration Range Sulfacetamide, sulfadiazine, sulfathiazole, sulfamerazine, sulfamethoxipyridazina, sulfamethazine, sulfaclorpyridazina, sulfamethoxazole, sulfaquinoxaline, sulfadimethoxine may be detected in liver of cattle, sheep, horses and pigs at ≥ 50 µg kg-1 concentration level.
QC Requirements See attached SOP
Method Source The Dr. Miguel C. Rubino Laboratory, Veterinary Laboratories Division (DILAVE), Ministry of Agriculture, Livestock and Fisheries, Republic of Uruguay
Method SOP PDF icon SOP 
Citation Citation