Veterinary Drugs Residue Methods


Category Beta-agonists
Drug Class Name Ractopamine, Phenethanolamine Beta-adrenergic agonist
Method Title Determination of Ractopamine Hydrochloride by High Performance Liquid Chromatography
Method Date 2004/07/06
Method Type Screening, Quantitative
Scope and Application The method is suitable for the determination of ractopamine in swine and bovine liver and muscle.
Method Summary Ground/blended samples are homogenized in methanol and extracted repeatedly by centrifugation. The volume of the supernatant is reduced by evaporation before adding borate buffer. This is mixed rigorously before adding ethyl acetate followed by centrifugation. The upper ethyl acetate layer following after repeated centrifugation is transferred into clean test tubes ready for solid phase extraction. Here an acidic alumina cartridge wetted with ethyl acetate is loading with the extract above. Ractopmaine is then eluted using methanol, which is then evaporated to dryness. The residue is dissolved in a sample diluent (2 percent aqueous acetic acid) and then pressed through a 0.22 micrometer filter prior to HPLC-fluorescence analysis where a C18 analytical column used for separation.
Applicable Concentration Range Ractopamine may be detected in swine and bovine liver and muscle tissues within the concentration ranges of 25 - 300 µg kg-1 and 1.0 - 300 µg kg-1, respectively.
QC Requirements See attached SOP
Method Source USDA-FSIS
Method SOP PDF icon SOP