Stable Isotope Reference Laboratory

Excel Spreadsheet for calculating isotopic abundances and δ2H, δ17O, δ18O values of water mixtures

Isotopic abundances and delta-values of water mixtures can be calculated for up to 10 waters of different isotopic compositions. Five of these constituents may consist of isotopically enriched water samples and tremendously facilitate mixing calculations. The spreadsheet is implemented in Microsoft Excel and was thoroughly tested and validated.

Related publications:

V. Faghihi, H.A.J Meijer, M. Gröning: A thoroughly validated spreadsheet for calculating isotopic abundances (2H, 17O, 18O) for mixtures of waters with different isotopic compositions. Rapid Communications in Mass Spectrometry (accepted for publication 2015)

V. Faghihi, B. M. A. A. Verstappen-Dumoulin, H. G. Jansen, G. van Dijk, A. T. Aerts-Bijma, E. R. T. Kerstel, M. Gröning and H. A. J. Meijer (2015): A new high-quality set of singly (2H) and doubly (2H and 18O) stable isotope labeled reference waters for biomedical and other isotope-labeled research. Rapid Communications in Mass Spectrometry, Volume 29, Issue 4, 28 February 2015, Pages: 311–321. Free download under:

Water Stable Isotope Calibration Program SICalib (version 2.14)

SICalib Program – Water Stable Isotope Calibration Program SICalib (version 2.14) is a macro based MS Excel spreadsheet program to evaluate raw data of water stable isotope measurements (δ2H and δ18O) as produced by a variety of instruments of different manufacturers. The main aim is to use consistent algorithms for data evaluation including corrections of memory and drift effects, for calibration and normalization and for calculation of combined standard uncertainty of all individual measurement results. The program was tested under MS Excel versions 2000, 2003, 2007 and 2010. Supported raw data include those produced by Thermo Finnigan ISODAT NT and 1.6 (for dual inlet mass spectrometry and water/gas equilibration), by Elementar/Isoprime MassLynx (continuous flow mass spectrometry and pyrolysis), and those by infrared laser spectroscopy based systems from manufacturers LGR and Picarro. A generic file format can be used to import other data formats as used by other manufacturers or system configurations.

The zipped file contains all necessary files, which should be unzipped to any directory of choice on a harddisk or USB-Drive. After having studied the supplied PDF-Manual, the data evaluation can be started by opening the main file SICalib.xls in MS Excel and choosing the appropriate data format.

Two other available zipped files contain executable files, which indeed are several minute long compressed video & audio sequences explaining special features of the use of SICalib.

SICalib-Videos1 – Several executable files, each containing largely compressed screenshot videos & audio sequences to explain features of SICalib.

SICalib-Videos2 - Several more executable files, each containing largely compressed screenshot videos & audio sequences to explain features of SICalib.


CalibTemplate – Zipped Excel Workbook, containing a calibration template for copy&paste of corrected raw data to perform a calibration, normalization and uncertainty calculation for measured water stable isotope data (δ2H and δ18O)

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